• Carmen Leitch - A Mysterious Plasmid is Found at High Levels in the Human Gut:

    https://www.labroots.com/trending/genetics-and-genomics/27029/mysterious-plasmid-found-levels-human-gut

    #Plasmid #PBI143 #Metagenome #Gastrointestinal #Gut #Microbiology #Biology
    Carmen Leitch - A Mysterious Plasmid is Found at High Levels in the Human Gut: https://www.labroots.com/trending/genetics-and-genomics/27029/mysterious-plasmid-found-levels-human-gut #Plasmid #PBI143 #Metagenome #Gastrointestinal #Gut #Microbiology #Biology
    WWW.LABROOTS.COM
    A Mysterious Plasmid is Found at High Levels in the Human Gut | Genetics And Genomics
    There are trillions of microorganisms in the human gastrointestinal tract, and this gut microbiome includes bacteria, archaea, viruses, and fungi. | Genetics And Genomics
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  • Stop All Nanoparticle CV19 Vax Injections Now – Karen Kingston
    https://rumble.com/v4jit9h-stop-all-nanoparticle-cv19-vax-injections-now-karen-kingston.html

    Karen Kingston is a biotech analyst and former Pfizer employee who has been reporting on the nightmare of the CV19 mRNA nanoparticle vax. Kingston proved the CV19 vax was a bioweapon from the very beginning and is now on a team pushing to stop using it in Florida. Kingston says, “We know the mRNA injections never provided any benefit, and because of that, they actually meet the definition of a biological weapon under 18 U.S. Code 175. It’s also a weapon of mass destruction under Florida law 790.166. The statement of facts that was submitted (to the Florida Supreme Court) goes through all the evidence. . . . There was 1.5 million adverse events. The definition of a weapon is any biological agent or device that doesn’t prevent against infection, doesn’t prevent any kind of disease . . . and was not done under bona fide research. We know that research that was done with the Phase 1, 2 and 3 trials, the Emergency Use Authorization (EUA) that was given to Americans and the FDA approved product, none of that was legitimate. There was no informed consent. That is criminal biological human experimentation. That, by definition, is biowarfare. There is no benefit . . . . It actually harms people. If you look at Pfizer’s documents, Moderna’s documents and the government’s documents, they knew all along that harms outweighed the benefits of this product in any population.”

    Dr. Joe Sansone in Florida has been the driving force to get these CV19 mRNA nanoparticle shots banned in that state. A recent post on Kingston’s Substack says, “Dr. Sansone is seeking for the Florida Supreme Court to issue an order to Governor DeSantis and Attorney General Moody to uphold their duties of enforcing federal and Florida laws by prohibiting the unlawful and criminal distribution, promotion and administration of the engineered mRNA nanoparticle injections on Floridians and to protect Floridians by seizing the injections.”

    (Read the entire “Order to Prohibit the Use Of and Seize the mRNA Injections” here.)

    Dr. Sansone is not the only one who thinks these CV19 injections should be “banned.” Kingston says, “The Florida Surgeon General and the Florida Department of Health have said these mRNA (CV19) injections should be used in NO human beings. They said these are gene editing technologies that can disrupt the human genome and make permanent changes. . . . According to the DOD, our own government and national security agencies say if you change the genome of any species, including human beings, that is the extermination of that species. This makes it a weapon of mass destruction. . . . The evidence is put together in such a damning way you cannot argue to keep the (CV19 mRNA nanoparticle) shots.”

    In closing, Kingston reminds us, “States have the right to block any drug or medical device even if the FDA approves it.” In the not-so-distant future, let’s hope many states follow Florida’s lead and ban the CV19 bioweapon shots.
    Stop All Nanoparticle CV19 Vax Injections Now – Karen Kingston https://rumble.com/v4jit9h-stop-all-nanoparticle-cv19-vax-injections-now-karen-kingston.html Karen Kingston is a biotech analyst and former Pfizer employee who has been reporting on the nightmare of the CV19 mRNA nanoparticle vax. Kingston proved the CV19 vax was a bioweapon from the very beginning and is now on a team pushing to stop using it in Florida. Kingston says, “We know the mRNA injections never provided any benefit, and because of that, they actually meet the definition of a biological weapon under 18 U.S. Code 175. It’s also a weapon of mass destruction under Florida law 790.166. The statement of facts that was submitted (to the Florida Supreme Court) goes through all the evidence. . . . There was 1.5 million adverse events. The definition of a weapon is any biological agent or device that doesn’t prevent against infection, doesn’t prevent any kind of disease . . . and was not done under bona fide research. We know that research that was done with the Phase 1, 2 and 3 trials, the Emergency Use Authorization (EUA) that was given to Americans and the FDA approved product, none of that was legitimate. There was no informed consent. That is criminal biological human experimentation. That, by definition, is biowarfare. There is no benefit . . . . It actually harms people. If you look at Pfizer’s documents, Moderna’s documents and the government’s documents, they knew all along that harms outweighed the benefits of this product in any population.” Dr. Joe Sansone in Florida has been the driving force to get these CV19 mRNA nanoparticle shots banned in that state. A recent post on Kingston’s Substack says, “Dr. Sansone is seeking for the Florida Supreme Court to issue an order to Governor DeSantis and Attorney General Moody to uphold their duties of enforcing federal and Florida laws by prohibiting the unlawful and criminal distribution, promotion and administration of the engineered mRNA nanoparticle injections on Floridians and to protect Floridians by seizing the injections.” (Read the entire “Order to Prohibit the Use Of and Seize the mRNA Injections” here.) Dr. Sansone is not the only one who thinks these CV19 injections should be “banned.” Kingston says, “The Florida Surgeon General and the Florida Department of Health have said these mRNA (CV19) injections should be used in NO human beings. They said these are gene editing technologies that can disrupt the human genome and make permanent changes. . . . According to the DOD, our own government and national security agencies say if you change the genome of any species, including human beings, that is the extermination of that species. This makes it a weapon of mass destruction. . . . The evidence is put together in such a damning way you cannot argue to keep the (CV19 mRNA nanoparticle) shots.” In closing, Kingston reminds us, “States have the right to block any drug or medical device even if the FDA approves it.” In the not-so-distant future, let’s hope many states follow Florida’s lead and ban the CV19 bioweapon shots.
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  • Oleg Sherbakov - Physicists model chromosome folding, reveal how loops affect spatial organization of the genome:

    https://phys.org/news/2023-11-physicists-chromosome-reveal-loops-affect.html

    #ChromosomalLoop #ChromosomeFolding #Chromosome #DNAFolding #DNA #LoopExtrusion #Topology #HumanGenome #Genome #PolymerPhysics #Polymer #Physics #Genomics #Biology
    Oleg Sherbakov - Physicists model chromosome folding, reveal how loops affect spatial organization of the genome: https://phys.org/news/2023-11-physicists-chromosome-reveal-loops-affect.html #ChromosomalLoop #ChromosomeFolding #Chromosome #DNAFolding #DNA #LoopExtrusion #Topology #HumanGenome #Genome #PolymerPhysics #Polymer #Physics #Genomics #Biology
    PHYS.ORG
    Physicists model chromosome folding, reveal how loops affect spatial organization of the genome
    Human chromosomes are long polymer chains that store genetic information. The nucleus of each cell contains the entire human genome (DNA) encoded on 46 chromosomes with a total length of about 2 meters. To fit into the microscopic cell nucleus and at the same time provide constant access to genetic information, chromosomes are folded in the nucleus in a special, predetermined way. DNA folding is an urgent task at the intersection of polymer physics and systems biology.
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  • Alarming COVID Jab Contamination Shocks Scientists - Dr Mercola
    https://www.youtube.com/watch?v=IEWHhrHiiTY

    Story at-a-glance

    In early April 2023, microbiologist Kevin McKernan detailed finding massive DNA contamination in Pfizer’s and Moderna’s bivalent COVID booster shots. The highest level of DNA contamination found was 30%, meaning nearly one-third of the content of the shot was plasmid DNA, the presence of which dramatically increases the likelihood of DNA integration and cancer
    An in vitro experiment found that the modified RNA in the Pfizer jab has the ability to enter human liver cells and reverse transcribe into DNA in as little as six hours post-exposure
    The lipid nanoparticles that the mRNA and DNA contaminants are encased in facilitate getting the DNA inside the cell. Once it’s in the cytoplasm, bits of DNA can enter the nucleus by random chance
    Mice injected with the COVID mRNA shot passed on their acquired immune traits — both good and bad — to offspring, which not only suggests that the mRNA can enter the nucleus of the cell, but also that it can be permanently integrated into chromosomal DNA and have intergenerational effects
    Pfizer’s bivalent jab has also been found to contain simian virus 40 (SV40) promoter, an oncogenic piece of the virus’ DNA known to drive very aggressive gene expression. Combined with pieces of DNA, the presence of SV40 promoter make the risk of cancer all the more likely
    The SV40 promoter is a sequence used in gene therapy to drive DNA into the nucleus of cells. If the shots aren’t supposed to alter the human genome, why do they contain bits of DNA and an SV40 promoter that can drive that DNA into the nucleus?


    https://articles.mercola.com/sites/articles/archive/2023/10/03/covid-jab-contamination.aspx?ui=551eda515442ee5cc8f0c77cc67b3f3febd63142ec0dc6cef1d4521e5ea666eb&sd=20200307&cid_source=dnl&cid_medium=email&cid_content=art1HL&cid=20231003&foDate=false&mid=DM1472322&rid=1928022111
    Alarming COVID Jab Contamination Shocks Scientists - Dr Mercola https://www.youtube.com/watch?v=IEWHhrHiiTY Story at-a-glance In early April 2023, microbiologist Kevin McKernan detailed finding massive DNA contamination in Pfizer’s and Moderna’s bivalent COVID booster shots. The highest level of DNA contamination found was 30%, meaning nearly one-third of the content of the shot was plasmid DNA, the presence of which dramatically increases the likelihood of DNA integration and cancer An in vitro experiment found that the modified RNA in the Pfizer jab has the ability to enter human liver cells and reverse transcribe into DNA in as little as six hours post-exposure The lipid nanoparticles that the mRNA and DNA contaminants are encased in facilitate getting the DNA inside the cell. Once it’s in the cytoplasm, bits of DNA can enter the nucleus by random chance Mice injected with the COVID mRNA shot passed on their acquired immune traits — both good and bad — to offspring, which not only suggests that the mRNA can enter the nucleus of the cell, but also that it can be permanently integrated into chromosomal DNA and have intergenerational effects Pfizer’s bivalent jab has also been found to contain simian virus 40 (SV40) promoter, an oncogenic piece of the virus’ DNA known to drive very aggressive gene expression. Combined with pieces of DNA, the presence of SV40 promoter make the risk of cancer all the more likely The SV40 promoter is a sequence used in gene therapy to drive DNA into the nucleus of cells. If the shots aren’t supposed to alter the human genome, why do they contain bits of DNA and an SV40 promoter that can drive that DNA into the nucleus? https://articles.mercola.com/sites/articles/archive/2023/10/03/covid-jab-contamination.aspx?ui=551eda515442ee5cc8f0c77cc67b3f3febd63142ec0dc6cef1d4521e5ea666eb&sd=20200307&cid_source=dnl&cid_medium=email&cid_content=art1HL&cid=20231003&foDate=false&mid=DM1472322&rid=1928022111
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  • The Best of Biden!
    The best thing Biden has done for America is mutate the Democratic genome so that this generation of Democrats is the last generation of Democrats, ever!
    The Best of Biden! The best thing Biden has done for America is mutate the Democratic genome so that this generation of Democrats is the last generation of Democrats, ever!
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  • USDA approves production, sale of cultivated chicken in US
    https://www.youtube.com/watch?v=eKhHQjRm3v4

    https://articles.mercola.com/sites/articles/archive/2023/07/13/pig-beans-gmo-frankenfood.aspx?ui=551eda515442ee5cc8f0c77cc67b3f3febd63142ec0dc6cef1d4521e5ea666eb&sd=20200307&cid_source=dnl&cid_medium=email&cid_content=art1ReadMore&cid=20230713&mid=DM1432461&rid=1854366733
    Story at-a-glance

    One of the latest GMO Frankenfoods is Piggy Sooy, a soybean genetically engineered to contain pig protein. One or more undisclosed pig genes are spliced into conventional soya to create a soybean with 26.6% animal protein
    Moolec, the U.K.-based company that developed Piggy Sooy, is also working on developing a pea plant that produces beef protein. The company claims these transgenic hybrids will provide similar taste, texture and nutritional value as meat, without the high cost of cultured or lab-grown meat alternatives
    June 21, 2023, the U.S. Department of Agriculture authorized the sale of cell-cultivated chicken from Good Meat and Upside Foods. Both plan on rolling out their synthetic chicken to “high-end” restaurants across the U.S. first, while they scale up production
    Researchers have discovered that CRISPR-Cas gene editing wreaks havoc in the plant genome, causing several hundred unintended genetic changes to occur simultaneously “in a catastrophic event” that ripples across large parts of the genome
    Because these changes are impossible to predict, gene edited plants cannot be assumed safe without extensive testing

    USDA approves production, sale of cultivated chicken in US https://www.youtube.com/watch?v=eKhHQjRm3v4 https://articles.mercola.com/sites/articles/archive/2023/07/13/pig-beans-gmo-frankenfood.aspx?ui=551eda515442ee5cc8f0c77cc67b3f3febd63142ec0dc6cef1d4521e5ea666eb&sd=20200307&cid_source=dnl&cid_medium=email&cid_content=art1ReadMore&cid=20230713&mid=DM1432461&rid=1854366733 Story at-a-glance One of the latest GMO Frankenfoods is Piggy Sooy, a soybean genetically engineered to contain pig protein. One or more undisclosed pig genes are spliced into conventional soya to create a soybean with 26.6% animal protein Moolec, the U.K.-based company that developed Piggy Sooy, is also working on developing a pea plant that produces beef protein. The company claims these transgenic hybrids will provide similar taste, texture and nutritional value as meat, without the high cost of cultured or lab-grown meat alternatives June 21, 2023, the U.S. Department of Agriculture authorized the sale of cell-cultivated chicken from Good Meat and Upside Foods. Both plan on rolling out their synthetic chicken to “high-end” restaurants across the U.S. first, while they scale up production Researchers have discovered that CRISPR-Cas gene editing wreaks havoc in the plant genome, causing several hundred unintended genetic changes to occur simultaneously “in a catastrophic event” that ripples across large parts of the genome Because these changes are impossible to predict, gene edited plants cannot be assumed safe without extensive testing
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  • Corrupting the Image through Seed Wars - Patenting the Human Genome

    I'm not sure the human race will survive!
    The US has approved "Franken-meat" for human consumption!

    Which is nothing more than a lab grown tumor, they put on your plate!

    The human race CANNOT SURVIVE if the #Corporation we call #Government remains in control over our country!

    Because, quite simply, they are actively MURDERING AMERICANS!
    And pissing and moaning will NOT fix this!

    "Voting" for a different CORPORATE slave master will NOT fix anything either!
    BOTH PARTIES are conspiring to murder YOU!

    They get on television and pretend to argue....
    But behind closed doors they ARE both working against YOU!
    And against this country, and everyone in it!

    It will require arrests and #Violence!
    If you are not willing to become violent with people trying to murder you....
    Then you are going to go EXTINCT! It's that simple!

    There can be no "waiting it out" here....
    We either ACT, and take these #Criminals out...
    The Feds, the Courts, the Police etc.... Or we DIE!

    If you do not control your LOCAL GOVERNMENT and men with guns within that local government.... Then you are a sitting duck, waiting to be plucked!

    https://rumble.com/v23a5yg-corrupting-the-image-through-seed-wars-patenting-the-human-genome.html
    Corrupting the Image through Seed Wars - Patenting the Human Genome I'm not sure the human race will survive! The US has approved "Franken-meat" for human consumption! Which is nothing more than a lab grown tumor, they put on your plate! The human race CANNOT SURVIVE if the #Corporation we call #Government remains in control over our country! Because, quite simply, they are actively MURDERING AMERICANS! And pissing and moaning will NOT fix this! "Voting" for a different CORPORATE slave master will NOT fix anything either! BOTH PARTIES are conspiring to murder YOU! They get on television and pretend to argue.... But behind closed doors they ARE both working against YOU! And against this country, and everyone in it! It will require arrests and #Violence! If you are not willing to become violent with people trying to murder you.... Then you are going to go EXTINCT! It's that simple! There can be no "waiting it out" here.... We either ACT, and take these #Criminals out... The Feds, the Courts, the Police etc.... Or we DIE! If you do not control your LOCAL GOVERNMENT and men with guns within that local government.... Then you are a sitting duck, waiting to be plucked! https://rumble.com/v23a5yg-corrupting-the-image-through-seed-wars-patenting-the-human-genome.html
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  • CV19 Bioweapon/Vax Beginning of Transhumanism – Karen Kingston, Greg Hunter
    https://rumble.com/v2sk82q-cv19-bioweaponvax-just-beginning-of-transhumanism-karen-kingston.html

    Karen Kingston is a biotech analyst and former Pfizer employee who understands complicated medical and biological contracts. Kingston warns the mRNA technology in the CV19 bioweapon injections is just the beginning of forcing a transhuman agenda onto an unsuspecting public. If the technology (which is also called a “synthetic pathogen”) in the CV19 bioweapon/vax is not stopped, there would be “no flesh would be saved” as Jesus warned more than 2,000 years ago. In a new Substack post, Kingston contends mRNA technology is a weapon of mass destruction and explains, “When I put that headline up that mRNA technologies, Neuralink or Neural Lace, which is the injectable form of Neuralink, are both weapons of mass destruction and transhumanism, this is to do non-human genetic editing to the human genome as well as to integrate bio-digital technology with humans. Those are not my words. Those are Dr. Robert Malone’s words during a Glenn Beck interview. This is where he said mRNA technologies are a suite of technologies for the purposes of transhumanism. . . . The reason why I say these are weapons of mass destruction is James Clapper, who is a former Lt. General with the U.S. Airforce and served in the intelligence community under President Clinton, stated that the gene editing mRNA technology are weapons of mass destruction. Because once you make permanent changes to the to a species genome, that’s to the extinction of that species. It doesn’t matter if it is plants, animals or human beings themselves.”

    Kingston goes on to say, “What we are breaking today is Covid 19 and the Covid 19 injections have a lot of neurological harmful effects. That’s because they are neural weapons as well . . . That’s the breaking news here. These CV19 injections include the Neural Lace technology. . . .Even Covid itself was a nanotechnology attack. It was not a virus. It is this lipid nanoparticulate matter. . . . With Covid, people lost their sense of taste and smell. . . . That is a nervous system attack. So, that would have to be a neuroweapon attack. People also have brain fog and fatigue. These are neurological symptoms of being attacked with a neural weapon. So, not only are the CV19 injections bioweapons, chemical weapons and technology weapons, they are also neural weapons. . . . This is complete experimentation on civilians without informed consent, and what the technologies are and potential outcomes of those technologies is by definition biowarfare. It’s in violation of the Geneva Convention. It’s in violation of the bioweapons convention. It’s in violation of the international code for military justice. It’s in violation of the 5th and 14th Amendments. Pfizer, Moderna and the other manufacturers are very guilty of unleashing weapons of mass destruction on global civilians.”

    Kingston also talks about mRNA technology referred to as synthetic biology that dark powers want to put in your food, water and even the air you breathe. Kingston will tell you how to stay clear of this destructive technology, and how to fight it too by taking things like Ivermectin. Kingston also discusses the need for a mass wakeup to the transhuman agenda using synthetic biology and mRNA. Kingston thinks if enough people can wake up and understand what is happening, this is the best way to bring it to a stop.

    In closing, Kingston says, “This is really important for people to understand and that is synthetic biology is changing the entire landscape of our world. . . . When they hijack the photosynthesis process of plants, and that is what they are doing, instead of producing oxygen and glucose, the plant is producing these new proteins which are weaponized. We will decrease the oxygen levels of all current biological lifeforms. So, it’s destroying the environment. This is directed evolution and transhumanism. It’s not just a war against humanity, it’s a war against all of God’s creation and all biological lifeforms.”

    Maybe this is why Jesus said in Matthew 24:22, “And unless those days were shortened, no flesh would be saved; but for the elect’s sake those days will be shortened.”
    CV19 Bioweapon/Vax Beginning of Transhumanism – Karen Kingston, Greg Hunter https://rumble.com/v2sk82q-cv19-bioweaponvax-just-beginning-of-transhumanism-karen-kingston.html Karen Kingston is a biotech analyst and former Pfizer employee who understands complicated medical and biological contracts. Kingston warns the mRNA technology in the CV19 bioweapon injections is just the beginning of forcing a transhuman agenda onto an unsuspecting public. If the technology (which is also called a “synthetic pathogen”) in the CV19 bioweapon/vax is not stopped, there would be “no flesh would be saved” as Jesus warned more than 2,000 years ago. In a new Substack post, Kingston contends mRNA technology is a weapon of mass destruction and explains, “When I put that headline up that mRNA technologies, Neuralink or Neural Lace, which is the injectable form of Neuralink, are both weapons of mass destruction and transhumanism, this is to do non-human genetic editing to the human genome as well as to integrate bio-digital technology with humans. Those are not my words. Those are Dr. Robert Malone’s words during a Glenn Beck interview. This is where he said mRNA technologies are a suite of technologies for the purposes of transhumanism. . . . The reason why I say these are weapons of mass destruction is James Clapper, who is a former Lt. General with the U.S. Airforce and served in the intelligence community under President Clinton, stated that the gene editing mRNA technology are weapons of mass destruction. Because once you make permanent changes to the to a species genome, that’s to the extinction of that species. It doesn’t matter if it is plants, animals or human beings themselves.” Kingston goes on to say, “What we are breaking today is Covid 19 and the Covid 19 injections have a lot of neurological harmful effects. That’s because they are neural weapons as well . . . That’s the breaking news here. These CV19 injections include the Neural Lace technology. . . .Even Covid itself was a nanotechnology attack. It was not a virus. It is this lipid nanoparticulate matter. . . . With Covid, people lost their sense of taste and smell. . . . That is a nervous system attack. So, that would have to be a neuroweapon attack. People also have brain fog and fatigue. These are neurological symptoms of being attacked with a neural weapon. So, not only are the CV19 injections bioweapons, chemical weapons and technology weapons, they are also neural weapons. . . . This is complete experimentation on civilians without informed consent, and what the technologies are and potential outcomes of those technologies is by definition biowarfare. It’s in violation of the Geneva Convention. It’s in violation of the bioweapons convention. It’s in violation of the international code for military justice. It’s in violation of the 5th and 14th Amendments. Pfizer, Moderna and the other manufacturers are very guilty of unleashing weapons of mass destruction on global civilians.” Kingston also talks about mRNA technology referred to as synthetic biology that dark powers want to put in your food, water and even the air you breathe. Kingston will tell you how to stay clear of this destructive technology, and how to fight it too by taking things like Ivermectin. Kingston also discusses the need for a mass wakeup to the transhuman agenda using synthetic biology and mRNA. Kingston thinks if enough people can wake up and understand what is happening, this is the best way to bring it to a stop. In closing, Kingston says, “This is really important for people to understand and that is synthetic biology is changing the entire landscape of our world. . . . When they hijack the photosynthesis process of plants, and that is what they are doing, instead of producing oxygen and glucose, the plant is producing these new proteins which are weaponized. We will decrease the oxygen levels of all current biological lifeforms. So, it’s destroying the environment. This is directed evolution and transhumanism. It’s not just a war against humanity, it’s a war against all of God’s creation and all biological lifeforms.” Maybe this is why Jesus said in Matthew 24:22, “And unless those days were shortened, no flesh would be saved; but for the elect’s sake those days will be shortened.”
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  • Because, by a strange twist in the story, her work was funded by the National Institute of Allergy and Infectious Diseases (NIAID), a part of the US National Institutes of Health (NIH). And grant proposals that funded her work, which are a matter of public record, specify exactly what she planned to do with the money.

    The grants were assigned to the prime contractor, Daszak of the EcoHealth Alliance, who subcontracted them to Shi. Here are extracts from the grants for fiscal years 2018 and 2019. (“CoV” stands for coronavirus and “S protein” refers to the virus’s spike protein.)

    “Test predictions of CoV inter-species transmission. Predictive models of host range (i.e. emergence potential) will be tested experimentally using reverse genetics, pseudovirus and receptor binding assays, and virus infection experiments across a range of cell cultures from different species and humanized mice.”

    “We will use S protein sequence data, infectious clone technology, in vitro and in vivo infection experiments and analysis of receptor binding to test the hypothesis that % divergence thresholds in S protein sequences predict spillover potential.”

    What this means, in non-technical language, is that Shi set out to create novel coronaviruses with the highest possible infectivity for human cells. Her plan was to take genes that coded for spike proteins possessing a variety of measured affinities for human cells, ranging from high to low. She would insert these spike genes one by one into the backbone of a number of viral genomes (“reverse genetics” and “infectious clone technology”), creating a series of chimeric viruses. These chimeric viruses would then be tested for their ability to attack human cell cultures (“in vitro”) and humanized mice (“in vivo”). And this information would help predict the likelihood of “spillover,” the jump of a coronavirus from bats to people.

    The methodical approach was designed to find the best combination of coronavirus backbone and spike protein for infecting human cells. The approach could have generated SARS2-like viruses, and indeed may have created the SARS2 virus itself with the right combination of virus backbone and spike protein.

    It cannot yet be stated that Shi did or did not generate SARS2 in her lab because her records have been sealed, but it seems she was certainly on the right track to have done so. “It is clear that the Wuhan Institute of Virology was systematically constructing novel chimeric coronaviruses and was assessing their ability to infect human cells and human-ACE2-expressing mice,” says Richard H. Ebright, a molecular biologist at Rutgers University and leading expert on biosafety. #Covid
    https://thebulletin.org/2021/05/the-origin-of-covid-did-people-or-nature-open-pandoras-box-at-wuhan/
    Because, by a strange twist in the story, her work was funded by the National Institute of Allergy and Infectious Diseases (NIAID), a part of the US National Institutes of Health (NIH). And grant proposals that funded her work, which are a matter of public record, specify exactly what she planned to do with the money. The grants were assigned to the prime contractor, Daszak of the EcoHealth Alliance, who subcontracted them to Shi. Here are extracts from the grants for fiscal years 2018 and 2019. (“CoV” stands for coronavirus and “S protein” refers to the virus’s spike protein.) “Test predictions of CoV inter-species transmission. Predictive models of host range (i.e. emergence potential) will be tested experimentally using reverse genetics, pseudovirus and receptor binding assays, and virus infection experiments across a range of cell cultures from different species and humanized mice.” “We will use S protein sequence data, infectious clone technology, in vitro and in vivo infection experiments and analysis of receptor binding to test the hypothesis that % divergence thresholds in S protein sequences predict spillover potential.” What this means, in non-technical language, is that Shi set out to create novel coronaviruses with the highest possible infectivity for human cells. Her plan was to take genes that coded for spike proteins possessing a variety of measured affinities for human cells, ranging from high to low. She would insert these spike genes one by one into the backbone of a number of viral genomes (“reverse genetics” and “infectious clone technology”), creating a series of chimeric viruses. These chimeric viruses would then be tested for their ability to attack human cell cultures (“in vitro”) and humanized mice (“in vivo”). And this information would help predict the likelihood of “spillover,” the jump of a coronavirus from bats to people. The methodical approach was designed to find the best combination of coronavirus backbone and spike protein for infecting human cells. The approach could have generated SARS2-like viruses, and indeed may have created the SARS2 virus itself with the right combination of virus backbone and spike protein. It cannot yet be stated that Shi did or did not generate SARS2 in her lab because her records have been sealed, but it seems she was certainly on the right track to have done so. “It is clear that the Wuhan Institute of Virology was systematically constructing novel chimeric coronaviruses and was assessing their ability to infect human cells and human-ACE2-expressing mice,” says Richard H. Ebright, a molecular biologist at Rutgers University and leading expert on biosafety. #Covid https://thebulletin.org/2021/05/the-origin-of-covid-did-people-or-nature-open-pandoras-box-at-wuhan/
    THEBULLETIN.ORG
    The origin of COVID: Did people or nature open Pandora’s box at Wuhan?
    If the case that SARS2 originated in a lab is so substantial, why isn’t this more widely known? As is now obvious, there are many people who have reason not to talk about it.
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