https://pubmed.ncbi.nlm.nih.gov/26814441/
Nanoscale
. 2016 Feb 14;8(6):3785-95. doi: 10.1039/c5nr09208f. Epub 2016 Jan 27.
Functionalized graphene oxide serves as a novel vaccine nano-adjuvant for robust stimulation of cellular immunity
Ligeng Xu 1, Jian Xiang, Ye Liu, Jun Xu, Yinchan Luo, Liangzhu Feng, Zhuang Liu, Rui Peng
Affiliations collapse
Affiliation
1Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou, 215123, P.R. China. [email protected] [email protected].
PMID: 26814441 DOI: 10.1039/c5nr09208f
Free article
Abstract
Benefiting from their unique physicochemical properties, graphene derivatives have attracted great attention in biomedicine. In this study, we carefully engineered graphene oxide (GO) as a vaccine adjuvant for immunotherapy using urease B (Ure B) as the model antigen. Ure B is a specific antigen for Helicobacter pylori, which is a class I carcinogen for gastric cancer. Polyethylene glycol (PEG) and various types of polyethylenimine (PEI) were used as coating polymers. Compared with single-polymer modified GOs (GO-PEG and GO-PEI), certain dual-polymer modified GOs (GO-PEG-PEI) can act as a positive modulator to promote the maturation of dendritic cells (DCs) and enhance their cytokine secretion through the activation of multiple toll-like receptor (TLR) pathways while showing low toxicity. Moreover, this GO-PEG-PEI can serve as an antigen carrier to effectively shuttle antigens into DCs. These two advantages enable GO-PEG-PEI to serve as a novel vaccine adjuvant. In the subsequent in vivo experiments, compared with free Ure B and clinically used aluminum-adjuvant-based vaccine (Alum-Ure B), GO-PEG-PEI-Ure B induces stronger cellular immunity via intradermal administration, suggesting promising applications in cancer immunotherapy. Our work not only presents a novel, highly effective GO-based vaccine nano-adjuvant, but also highlights the critical roles of surface chemistry for the rational design of nano-adjuvants.
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Nanoscale
. 2016 Feb 14;8(6):3785-95. doi: 10.1039/c5nr09208f. Epub 2016 Jan 27.
Functionalized graphene oxide serves as a novel vaccine nano-adjuvant for robust stimulation of cellular immunity
Ligeng Xu 1, Jian Xiang, Ye Liu, Jun Xu, Yinchan Luo, Liangzhu Feng, Zhuang Liu, Rui Peng
Affiliations collapse
Affiliation
1Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou, 215123, P.R. China. [email protected] [email protected].
PMID: 26814441 DOI: 10.1039/c5nr09208f
Free article
Abstract
Benefiting from their unique physicochemical properties, graphene derivatives have attracted great attention in biomedicine. In this study, we carefully engineered graphene oxide (GO) as a vaccine adjuvant for immunotherapy using urease B (Ure B) as the model antigen. Ure B is a specific antigen for Helicobacter pylori, which is a class I carcinogen for gastric cancer. Polyethylene glycol (PEG) and various types of polyethylenimine (PEI) were used as coating polymers. Compared with single-polymer modified GOs (GO-PEG and GO-PEI), certain dual-polymer modified GOs (GO-PEG-PEI) can act as a positive modulator to promote the maturation of dendritic cells (DCs) and enhance their cytokine secretion through the activation of multiple toll-like receptor (TLR) pathways while showing low toxicity. Moreover, this GO-PEG-PEI can serve as an antigen carrier to effectively shuttle antigens into DCs. These two advantages enable GO-PEG-PEI to serve as a novel vaccine adjuvant. In the subsequent in vivo experiments, compared with free Ure B and clinically used aluminum-adjuvant-based vaccine (Alum-Ure B), GO-PEG-PEI-Ure B induces stronger cellular immunity via intradermal administration, suggesting promising applications in cancer immunotherapy. Our work not only presents a novel, highly effective GO-based vaccine nano-adjuvant, but also highlights the critical roles of surface chemistry for the rational design of nano-adjuvants.
Similar articles
Alum-functionalized graphene oxide nanocomplexes for effective anticancer vaccination.
Wang X, Cao F, Yan M, Liu Y, Zhu X, Sun H, Ma G.
Acta Biomater. 2019 Jan 1;83:390-399. doi: 10.1016/j.actbio.2018.11.023. Epub 2018 Nov 15.
PMID: 30448435
Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages.
Wang B, Su X, Liang J, Yang L, Hu Q, Shan X, Wan J, Hu Z.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:514-522. doi: 10.1016/j.msec.2018.04.096. Epub 2018 May 1.
PMID: 29853120
Immunostimulatory oligonucleotides-loaded cationic graphene oxide with photothermally enhanced immunogenicity for photothermal/immune cancer therapy.
Tao Y, Ju E, Ren J, Qu X.
Biomaterials. 2014 Dec;35(37):9963-9971. doi: 10.1016/j.biomaterials.2014.08.036. Epub 2014 Sep 15.
PMID: 25224368
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Cao W, He L, Cao W, Huang X, Jia K, Dai J.
Acta Biomater. 2020 Aug;112:14-28. doi: 10.1016/j.actbio.2020.06.009. Epub 2020 Jun 10.
PMID: 32531395 Review.
Particulate formulations for the delivery of poly(I:C) as vaccine adjuvant.
Hafner AM, Corthésy B, Merkle HP.
Adv Drug Deliv Rev. 2013 Oct;65(10):1386-99. doi: 10.1016/j.addr.2013.05.013. Epub 2013 Jun 7.
PMID: 23751781 Review.
See all similar articles
Cited by 7 articles
Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo- and heterologous strains.
Dong C, Wang Y, Gonzalez GX, Ma Y, Song Y, Wang S, Kang SM, Compans RW, Wang BZ.
Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2024998118. doi: 10.1073/pnas.2024998118.
PMID: 33941704 Free PMC article.
SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β.
Liu H, Dong H, Zhou N, Dong S, Chen L, Zhu Y, Hu HM, Mou Y.
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PMID: 30570682 Free PMC article.
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Chattopadhyay S, Chen JY, Chen HW, Hu CJ.
Nanotheranostics. 2017 Jun 9;1(3):244-260. doi: 10.7150/ntno.19796. eCollection 2017.
PMID: 29071191 Free PMC article. Review.
Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells.
Orecchioni M, Bedognetti D, Newman L, Fuoco C, Spada F, Hendrickx W, Marincola FM, Sgarrella F, Rodrigues AF, Ménard-Moyon C, Cesareni G, Kostarelos K, Bianco A, Delogu LG.
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Front Immunol. 2017 Jun 13;8:673. doi: 10.3389/fimmu.2017.00673. eCollection 2017.
https://pubmed.ncbi.nlm.nih.gov/26814441/
Nanoscale
. 2016 Feb 14;8(6):3785-95. doi: 10.1039/c5nr09208f. Epub 2016 Jan 27.
Functionalized graphene oxide serves as a novel vaccine nano-adjuvant for robust stimulation of cellular immunity
Ligeng Xu 1, Jian Xiang, Ye Liu, Jun Xu, Yinchan Luo, Liangzhu Feng, Zhuang Liu, Rui Peng
Affiliations collapse
Affiliation
1Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou, 215123, P.R. China. [email protected] [email protected].
PMID: 26814441 DOI: 10.1039/c5nr09208f
Free article
Abstract
Benefiting from their unique physicochemical properties, graphene derivatives have attracted great attention in biomedicine. In this study, we carefully engineered graphene oxide (GO) as a vaccine adjuvant for immunotherapy using urease B (Ure B) as the model antigen. Ure B is a specific antigen for Helicobacter pylori, which is a class I carcinogen for gastric cancer. Polyethylene glycol (PEG) and various types of polyethylenimine (PEI) were used as coating polymers. Compared with single-polymer modified GOs (GO-PEG and GO-PEI), certain dual-polymer modified GOs (GO-PEG-PEI) can act as a positive modulator to promote the maturation of dendritic cells (DCs) and enhance their cytokine secretion through the activation of multiple toll-like receptor (TLR) pathways while showing low toxicity. Moreover, this GO-PEG-PEI can serve as an antigen carrier to effectively shuttle antigens into DCs. These two advantages enable GO-PEG-PEI to serve as a novel vaccine adjuvant. In the subsequent in vivo experiments, compared with free Ure B and clinically used aluminum-adjuvant-based vaccine (Alum-Ure B), GO-PEG-PEI-Ure B induces stronger cellular immunity via intradermal administration, suggesting promising applications in cancer immunotherapy. Our work not only presents a novel, highly effective GO-based vaccine nano-adjuvant, but also highlights the critical roles of surface chemistry for the rational design of nano-adjuvants.
Similar articles
Alum-functionalized graphene oxide nanocomplexes for effective anticancer vaccination.
Wang X, Cao F, Yan M, Liu Y, Zhu X, Sun H, Ma G.
Acta Biomater. 2019 Jan 1;83:390-399. doi: 10.1016/j.actbio.2018.11.023. Epub 2018 Nov 15.
PMID: 30448435
Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages.
Wang B, Su X, Liang J, Yang L, Hu Q, Shan X, Wan J, Hu Z.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:514-522. doi: 10.1016/j.msec.2018.04.096. Epub 2018 May 1.
PMID: 29853120
Immunostimulatory oligonucleotides-loaded cationic graphene oxide with photothermally enhanced immunogenicity for photothermal/immune cancer therapy.
Tao Y, Ju E, Ren J, Qu X.
Biomaterials. 2014 Dec;35(37):9963-9971. doi: 10.1016/j.biomaterials.2014.08.036. Epub 2014 Sep 15.
PMID: 25224368
Recent progress of graphene oxide as a potential vaccine carrier and adjuvant.
Cao W, He L, Cao W, Huang X, Jia K, Dai J.
Acta Biomater. 2020 Aug;112:14-28. doi: 10.1016/j.actbio.2020.06.009. Epub 2020 Jun 10.
PMID: 32531395 Review.
Particulate formulations for the delivery of poly(I:C) as vaccine adjuvant.
Hafner AM, Corthésy B, Merkle HP.
Adv Drug Deliv Rev. 2013 Oct;65(10):1386-99. doi: 10.1016/j.addr.2013.05.013. Epub 2013 Jun 7.
PMID: 23751781 Review.
See all similar articles
Cited by 7 articles
Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo- and heterologous strains.
Dong C, Wang Y, Gonzalez GX, Ma Y, Song Y, Wang S, Kang SM, Compans RW, Wang BZ.
Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2024998118. doi: 10.1073/pnas.2024998118.
PMID: 33941704 Free PMC article.
SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β.
Liu H, Dong H, Zhou N, Dong S, Chen L, Zhu Y, Hu HM, Mou Y.
Nanoscale Res Lett. 2018 Dec 20;13(1):409. doi: 10.1186/s11671-018-2802-0.
PMID: 30570682 Free PMC article.
Nanoparticle Vaccines Adopting Virus-like Features for Enhanced Immune Potentiation.
Chattopadhyay S, Chen JY, Chen HW, Hu CJ.
Nanotheranostics. 2017 Jun 9;1(3):244-260. doi: 10.7150/ntno.19796. eCollection 2017.
PMID: 29071191 Free PMC article. Review.
Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells.
Orecchioni M, Bedognetti D, Newman L, Fuoco C, Spada F, Hendrickx W, Marincola FM, Sgarrella F, Rodrigues AF, Ménard-Moyon C, Cesareni G, Kostarelos K, Bianco A, Delogu LG.
Nat Commun. 2017 Oct 24;8(1):1109. doi: 10.1038/s41467-017-01015-3.
PMID: 29061960 Free PMC article.
Graphene and the Immune System: A Romance of Many Dimensions.
Mukherjee SP, Bottini M, Fadeel B.
Front Immunol. 2017 Jun 13;8:673. doi: 10.3389/fimmu.2017.00673. eCollection 2017.
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